private const uint WS_EX_LAYERED = 0x80000;
private const int WS_EX_TRANSPARENT = 0x20;
private const int GWL_STYLE = (-16);
private const int GWL_EXSTYLE = (-20);
private const int LWA_ALPHA = 100;

[DllImport("user32", EntryPoint = "SetWindowLong")]
private static extern uint SetWindowLong(
IntPtr hwnd,
int nIndex,
uint dwNewLong
);

[DllImport("user32", EntryPoint = "GetWindowLong")]
private static extern uint GetWindowLong(
IntPtr hwnd,
int nIndex
);

[DllImport("user32", EntryPoint = "SetLayeredWindowAttributes")]
private static extern int SetLayeredWindowAttributes(
IntPtr hwnd,
int crKey,
int bAlpha,
int dwFlags
);

/// <summary> 
/// 设置窗体具有鼠标穿透效果 
/// </summary> 
public void SetPenetrate()
{
    this.TopMost = true;
    SetWindowLong(this.Handle, GWL_EXSTYLE, WS_EX_TRANSPARENT | WS_EX_LAYERED);
    SetLayeredWindowAttributes(this.Handle, 0, 100, LWA_ALPHA);
}

C#winform

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